Solution based genomic DNA purification from whole blood
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months
Availability Restriction: Exclusively distributed in Japan by Greiner BioOne
Blood DNA Preparation Kit is designed for convenient and fast isolation of genomic DNA from whole blood samples. The solution based system minimizes DNA fragmentation that may be problematic in spin-column / filtration based methods. Because phenol or chloroform is not used it is safe and does not produce any harmful waste.
Solution based genomic DNA purification kits guarantee minimal DNA fragmentation and yield DNA sized up to 150 kb.
Yields of genomic DNA will vary from sample to sample depending on the amount, quality and type of material processed. An amount of approx. 30-50 μg purified DNA can be expected per preparation of 300 μl whole blood. Upscaling of the preparation by a factor of 10 is easily possible if larger amounts of DNA are required.
RBC Lysis Solution
Cell Lysis Solution
Protein Precipitation Solution
Washing Buffer (before use, add 96-99 % Ethanol as indicated on the bottle)
DNA Hydration Solution
To be provided by you:
Isopropanol (2-propanol) >99 %
96-99 % Ethanol
Microtubes 1.5 or 2.0 ml
Heating Block or Water Bath at 65 °C
Before start, provide >99 % Isopropanol (2-propanol) (not included in the kit).
For XS pack (20 preps): Add 9.6 ml 96-99 % Ethanol (not included in the kit) to the Washing Buffer bottle.
For S pack (100 preps): Add 48 ml 96-99 % Ethanol (not included in the kit) to the Washing Buffer bottle.
For L pack (500 preps): Add 120 ml 96-99 % Ethanol (not included in the kit) to each Washing Buffer bottle.
|19.2 ml||96 ml||2x 240 ml|
|6.4 ml||32 ml||160 ml|
|2.2 ml||11 ml||55 ml|
|Washing Buffer||add 9.6 ml Ethanol |
(final volume 12 ml)
|add 48 ml Ethanol |
(final volume 60 ml)
|add 120 ml Ethanol to each bottle (final volume 150 ml each)|
|DNA Hydration Solution||2.2 ml||11 ml||55 ml|
1 Cell Lysis:
2 Protein Precipitation:
3 DNA Precipitation:
4 DNA Hydration: