Fast removal of unincorporated dye-nucleotides or dye-terminators
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months
Nucleotide/Dye Removal Kit is designed for fast and easy separation of unincorporated dye-labeled, marker-labeled or unlabeled nucleotides from DNA. The kit contains ready-to-use spin columns preloaded with a gel filtration resin. The simple cleanup procedure is based on gel filtration technology and guarantees highest recovery rates.
The kit has been optimized for cleanup of enzymatic DNA labeling reactions like PCR probe labeling, DNA sequencing, Nick Translation or DNA end-labeling. It allows the removal of labeled dNTPs or ddNTPs in a single step within a few minutes. The purified DNA is applicable in down-stream applications like FISH (fluorescence in situ hybridization) or Southern - / Northern blotting.
Recommended sample volume: 10-20 μl
Recommended centrifugal force: 1,000 g
DNA fragment cut-off: 20 bp
Gel Filtration Spin Columns
2 ml Collection Tubes
To be provided by you:
1.5-2 ml Microcentrifuge Tubes
1 Column set-up:
2 DNA cleanup: