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RNase Inhibitor

Inhibition of RNases by non-covalent binding

Product Cat. No. Amount Price (EUR) Buy / Note
RNase Inhibitor PCR-504S 2 kunits 45,00 Add to Basket/Quote Add to Notepad
RNase Inhibitor PCR-504L 10 kunits 180,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Unit Definition: One unit of the protein inhibits the activity of 5 ng RNase A by 50 %. Inhibitor activity is assayed in: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/ml BSA, 100 ng/ml RNase A, 0.1 mg/ml E.coli [3H]-RNA, 50 mg/ml yeast RNA and 8 mM DTT.

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 49.6 kDa monomer

Form: liquid (Supplied in 20 mM HEPES-KOH pH 7.5, 100 mM KCl, 0.1 mM EDTA, 2 mM DTT and 50 % [v/v] glycerol)

Concentration: 40 units/μl

Description:
RNase Inhibitor completely inhibits the activity of RNases A, B and C by non-covalent binding. It binds the RNases in a 1:1 ratio with an association constant of approx. 1014 M. It does not inhibit the RNases I, T1, T2, H, U1, U2 and CL3.

Applications:
RNase inhibitor should be applied in a working concentration of 40 units (1 μl) per 50 μl reaction volume. The application of RNase inhibitor is recommended for:

  • cDNA synthesis / RT-PCR
  • in vitro transcription
  • in vitro translation
  • isolation of mammalian cell fractions that contain mRNA-protein complex
  • Separation and identification of specific ribonuclease activities
  • Studies of tumor suppression

Availability Restriction: Exclusively distributed in Japan by Greiner BioOne