Inhibition of RNases by non-covalent binding
For in vitro use only!
Unit Definition: One unit of the protein inhibits the activity of 5 ng RNase A by 50 %. Inhibitor activity is assayed in: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/ml BSA, 100 ng/ml RNase A, 0.1 mg/ml E.coli [3H]-RNA, 50 mg/ml yeast RNA and 8 mM DTT.
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 49.6 kDa monomer
Form: liquid (Supplied in 20 mM HEPES-KOH pH 7.5, 100 mM KCl, 0.1 mM EDTA, 2 mM DTT and 50 % [v/v] glycerol)
Concentration: 40 units/μl
Availability Restriction: Exclusively distributed in Japan by Greiner BioOne
RNase Inhibitor completely inhibits the activity of RNases A, B and C by non-covalent binding. It binds the RNases in a 1:1 ratio with an association constant of approx. 1014 M. It does not inhibit the RNases I, T1, T2, H, U1, U2 and CL3.
RNase inhibitor should be applied in a working concentration of 40 units (1 μl) per 50 μl reaction volume. The application of RNase inhibitor is recommended for: