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RNase Inhibitor - cloned

mice cell line

Cat. No. Amount Price (EUR) Buy / Note
PCR-504S 2.000 units 57,61 Add to Basket/Quote Add to Notepad
PCR-504L 10.000 units 230,42 Add to Basket/Quote Add to Notepad

For in vitro use only!

Unit Definition: One unit of the protein inhibits the activity of 5 ng RNase A by 50 %. Inhibitor activity is assayed in: 40 mM Tris-HCl (pH 8.0, 25 °C), 10 mM MgSO4, 1 mM CaCl2.

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid (Supplied in 20 mM HEPES-KOH pH 7.5, 100 mM KCl, 0.1 mM EDTA and 50 % [v/v] glycerol)

Concentration: 40 units/μl

RNase inhibitor should be applied in a working concentration of 40 units (1 μl) per 50 μl reaction volume. The application of RNase inhibitor is recommended for:

  • cDNA synthesis / RT-PCR
  • in vitro transcription
  • in vitro translation
  • isolation of mammalian cell fractions that contain mRNA-protein complex
  • Separation and identification of specific ribonuclease activities
  • Studies of tumor suppression

RNase Inhibitor completely inhibits the activity of RNases A, B and C by non-covalent binding. It binds the RNases in a 1:1 ratio with an association constant of approx. 1014 M. It does not inhibit the RNases I, T1, T2, H, U1, U2 and CL3.

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