DNA intercalation dye for real-time PCR analysis
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Form: orange liquid (Supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20)
Concentration: 100 μM
Spectroscopic Properties: λexc 594 nm (bound to DNA), λem 520 nm (bound to DNA)
SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
Select the optical setting for SYBR® Green or FAM on the detection instrument to perform the assay.
SYBR® Green Fluorescent DNA Stain is supplied as 100 μM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 μM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors.
Select the optical setting for SYBR® Green or FAM on the detection instrument.
|final SYBR® Green concentration||20 μl PCR assay||50 μl PCR assay|
|0.5 μM||0.1 μl||0.25 μl|
|1.0 μM||0.2 μl||0.50 μl|
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