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Ready-to-Use Lyophilisates

Lyophilisates – Preloaded and Stable at Room Temperature

Ready-to-Use Lyophilisates are delivered in PCR reaction tube strips or 96-well plates preloaded with a complete master mix in a dry, room temperature stable format. The lyophilisates combine highest performance with convenience of use and stability. There is no need for freezing, thawing or pipetting on ice. The few remaining pipetting steps minimize the risk of errors or contaminations. Each vial contains enzyme, dNTPs and reaction buffer required for a 20 μl PCR assay. Simply fill up the vials with a premix of RNA template and primers and run the reverse transcripton in a thermocycler as usual.

Ready-to-Use Lyophilisates containing custom primers are custom made master mix lyophilisates comprising primers, enzyme, dNTPs and reaction buffer. Provide us with primer sequence and we deliver preloaded PCR tubes and plates ready-made for your special application. The only thing you still have to do is adding template RNA, filling up with water and starting the cycler!

Development, optimization and scale-up of custom-specific lyophilisates

Freeze-dryed mixes are stable at ambient temperatures and significantly reduce contamination risk, turnaround time and total costs.

Lyophilisation service of Jena Bioscience at a glance

Optimization

  • adaptation of in-house lyophilisates to customized applications
  • modification of liquid custom-established assays for lyophilisation
  • optimization of the lyophilisation process to improve product functionality, stability and appearance

Lyophilisation
  • addition of custom-synthesized primers and internal/positive control DNA
  • up-scaling from small batch sizes to several thousand assay per batch
  • highly efficient cryo- and lyoprotectors with minimal influence on PCR available

Packaging
  • lyophilisation of PCR mixes in single PCR tubes, 8-well tube strips, 96-well plates or custom-specific formats
  • thermosealing into aluminium composite foil according to individual demands
  • custom labeling

Ready-to-Use Lyophilisates containing custom primers are custom made master mix lyophilisates comprising primers, polymerase, dNTPs and reaction buffer. Provide us with primer sequence and we deliver preloaded PCR tubes and plates ready-made for your special application. The only thing you still have to do is adding template DNA, filling up with water and starting the cycler!