SCRIPT One-Step RT-qPCR Master Mixes allow quantitative mRNA detection by reverse transcription and real-time PCR. Reactions are carried out sequentially in a single tube, thus minimizing pipetting steps and contamination risk. Targets from <1 pg mRNA or 10 pg total RNA are detectable. All mixes are supplied as 2x concentrated solution containing all reagents required for RT-qPCR (except template and primers). Kits are available in combination with Dual Labeled Fluorescent Probes or EvaGreen™ fluorescent DNA stain.