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SYBR® Green Fluorescent DNA Stain

DNA intercalation dye for real-time PCR analysis

Cat. No. Amount Price (EUR) Buy / Note
PCR-378 500 μl x 100 μM 47,36 Add to Basket/Quote Add to Notepad
Excitation (blue) and emission (green) spectrum of SYBR® Green bound to dsDNA
Excitation (blue) and emission (green) spectrum of SYBR® Green bound to dsDNA

For in vitro use only!

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
store dark

Shelf Life: 12 months

Form: liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20

Color: orange

Concentration: 100 μM

Spectroscopic Properties: λexc = 495 nm, λem = 520 nm (bound to DNA)

Description:
SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.

SYBR® Green Fluorescent DNA Stain is supplied as 100 μM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 μM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors.
Select the optical setting for SYBR® Green or FAM on the detection instrument.

final SYBR® Green concentration20 μl PCR assay50 μl PCR assay
0.5 μM0.1 μl0.25 μl
1.0 μM0.2 μl0.50 μl

SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.

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