Saturating dsDNA-binding dye for real-time PCR and high-resolution melting analysis
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| PCR-379-1ML | 1 ml x 100 μM | 158,08 | Add to Basket/Quote Add to Notepad |
| PCR-379-10ML | 10 ml x 100 μM | 948,50 | Add to Basket/Quote Add to Notepad |
| PCR-379-100ML | 100 ml x 100 μM | Ask for Quotation |
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid repeated freeze-thaw cycles, aliquot if necessary
protect from light
Shelf Life: 12 months
Form: liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20
Color: orange (stock solution)
Concentration: 100 μM
Spectroscopic Properties: λexc = 500 nm, λem = 530 nm (bound to DNA); λexc = 471 nm (without DNA)
OD = 2 +/- 0.15 (λ = 469 nm, c = 25 μM in PBS buffer pH 7.4, without DNA)
Description:
InfiniteGreen Fluorescent DNA Stain is a saturating dsDNA-binding fluorescent dye developed as a functional alternative and replacement to EvaGreen® dye for real-time PCR (qPCR) and high-resolution melting (HRM) analysis. The dye shows strong fluorescence enhancement upon binding to double-stranded DNA and is designed for use in PCR-based DNA detection workflows. InfiniteGreen is particularly suitable for applications requiring saturating dye conditions, including HRM analysis, genotyping, mutation screening and melt curve-based amplicon analysis.
InfiniteGreen Fluorescent DNA Stain is supplied as a 100 μM solution. Vortex thoroughly before use. A final concentration of 1.0 - 1.5 μM is recommended. Optimal concentration may depend on the instrument, assay chemistry, amplicon length and PCR setup. For quantitative PCR, preparation of a master mix is recommended to reduce pipetting errors and improve reproducibility.
Recommended final concentration
Add InfiniteGreen Fluorescent DNA Stain to each reaction as indicated in the table below.
For instrument setup, use the optical channel commonly used for EvaGreen®, SYBR® Green or FAM detection.
| InfiniteGreen final concentration | 20 μl PCR assay | 50 μl PCR assay |
| 1.0 μM | 0.2 μl | 0.50 μl |
| 1.5 μM | 0.3 μl | 0.75 μl |
EvaGreen® is a registered trademark of Biotium, Inc.
SYBR® is a registered trademark of Molecular Probes, Inc.
All trademarks are the property of their respective owners.
BIOZ Product Citations:
Powered by Bioz
