InfiniteGreen Fluorescent DNA Stain
Excitation (black) and emission (green) spectrum of InfiniteGreen bound to dsDNA in PBS buffer (pH 7.3)
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
InfiniteGreen Fluorescent DNA Stain is a saturating dsDNA-binding fluorescent dye developed as a functional alternative and replacement to EvaGreen® dye for real-time PCR (qPCR) and high-resolution melting (HRM) analysis. The dye shows strong fluorescence enhancement upon binding to double-stranded DNA and is designed for use in PCR-based DNA detection workflows. InfiniteGreen is particularly suitable for applications requiring saturating dye conditions, including HRM analysis, genotyping, mutation screening and melt curve-based amplicon analysis.
InfiniteGreen Fluorescent DNA Stain is supplied as a 100 μM solution. Vortex thoroughly before use. A final concentration of 1.0 - 1.5 μM is recommended. Optimal concentration may depend on the instrument, assay chemistry, amplicon length and PCR setup. For quantitative PCR, preparation of a master mix is recommended to reduce pipetting errors and improve reproducibility.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid repeated freeze-thaw cycles, aliquot if necessary
protect from light
Shelf Life: 12 months
Form: liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20
Color: orange (stock solution)
Concentration: 100 μM
Spectroscopic Properties: λexc = 500 nm, λem = 530 nm (bound to DNA); λexc = 471 nm (without DNA)
OD = 2 +/- 0.15 (λ = 469 nm, c = 25 μM in PBS buffer pH 7.4, without DNA)
Recommended final concentration
Add InfiniteGreen Fluorescent DNA Stain to each reaction as indicated in the table below.
For instrument setup, use the optical channel commonly used for EvaGreen®, SYBR® Green or FAM detection.
| InfiniteGreen final concentration | 20 μl PCR assay | 50 μl PCR assay |
|---|---|---|
| 1.0 μM | 0.2 μl | 0.50 μl |
| 1.5 μM | 0.3 μl | 0.75 μl |
EvaGreen® is a registered trademark of Biotium, Inc.
SYBR® is a registered trademark of Molecular Probes, Inc.
All trademarks are the property of their respective owners.
| Cat. No. | Amount | Price |
|---|---|---|
| PCR-379-1ML | 1 ml x 100 μM | 110,66 € |
| PCR-379-10ML | 10 ml x 100 μM | 663,95 € |
| PCR-379-100ML | 100 ml x 100 μM | Ask for Quotation |
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Scientific citations for Jena Bioscience pcr-379
14 scientific citations and 0 citation images found by Bioz.
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Loop extrusion dynamics and cooperative activation independently regulate enhancer-driven transcription
bioRxiv, 2026-05-07 · DOI:10.64898/2026.05.06.723196
PrePrint: .. was performed using NEBNext UltraTM II DNA Library Prep Kit for Illumina (NEB, E7103).. Library PCR was performed using index primers (NEBNext Multiplex Oligos for Illumina, Dual Index Primers Set 1; NEB, E7600S) supplemented with 1x EvaGreen (Jena Bioscience,
PCR-379 -
Thermal-bias PCR: generation of amplicon libraries without degenerate primer interference
PeerJ, 2025-10-24 · DOI:10.7717/peerj.20241
.. and 1 μL was used per 20 μL of reaction mixture (97.5 pg/μL final).. Platinum II Taq and Ultra II Q5 reactions were supplemented with EvaGreen dye (
Jena Bioscience , cat. #PCR-379 -
Multiplexed detection of respiratory viral pathogens by isothermal amplification on an autonomously loaded chip at the point-of-care.
Lab on a Chip, 2025-10-23 · PMID:41128070
.. μL−1) and betaine (Cat. # J77507) were obtained from Thermo Fisher Scientific (Vilnius, Lithuania).. D-(+)-trehalose dehydrate was purchased from Merck (Germany, Cat. # 90210), mineral oil from Carl Roth (Karlsruhe, Germany, Cat. # HP50.1), and EvaGreen® from
Jena Bioscience (Jena, Germany, Cat. #PCR-379
