DNA intercalator dye for real-time PCR analysis
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| PCR-378-1ML | 1 ml x 100 μM | 105,20 | Add to Basket/Quote Add to Notepad |
| PCR-378-10ML | 10 ml x 100 μM | 526,00 | Add to Basket/Quote Add to Notepad |
| PCR-378-100ML | 100 ml x 100 μM | Ask for Quotation |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
store dark
Shelf Life: 12 months
Form: liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20
Color: orange
Concentration: 100 μM
Spectroscopic Properties: λexc 495 nm, λem 520 nm (bound to DNA)
Description:
Green-fluorescent DNA stain is structurally similar to SYBR® GREEN and a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
Green-Fluorescent DNA Stain is supplied as 100 μM concentration. Vortex Green-Fluorescent DNA Stain thoroughly prior to its use. An Green-Fluorescent DNA Stain concentration of 0.5-1.0 μM in the final assay is recommended. Add Green-Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors.
Select the optical setting for SYBR® GREEN or FAM on the detection instrument.
| final Green-Fluorescent DNA Stain concentration | 20 μl PCR assay | 50 μl PCR assay |
| 0.5 μM | 0.1 μl | 0.25 μl |
| 1.0 μM | 0.2 μl | 0.50 μl |
® SYBR is a trademark of Molecular Probes, Inc.
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