Green-Fluorescent DNA Stain
Excitation (blue) and emission (green) spectrum of green-fluorescent DNA stain bound to dsDNA
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
Green-fluorescent DNA stain is structurally similar to SYBR® GREEN and a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
store dark
Shelf Life: 12 months
Form: liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20
Color: orange
Concentration: 100 μM
Spectroscopic Properties: λexc 495 nm, λem 520 nm (bound to DNA)
Green-Fluorescent DNA Stain is supplied as 100 μM concentration. Vortex Green-Fluorescent DNA Stain thoroughly prior to its use. An Green-Fluorescent DNA Stain concentration of 0.5-1.0 μM in the final assay is recommended. Add Green-Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors.
Select the optical setting for SYBR® GREEN or FAM on the detection instrument.
| final Green-Fluorescent DNA Stain concentration | 20 μl PCR assay | 50 μl PCR assay |
|---|---|---|
| 0.5 μM | 0.1 μl | 0.25 μl |
| 1.0 μM | 0.2 μl | 0.50 μl |
® SYBR is a trademark of Molecular Probes, Inc.
| Cat. No. | Amount | Price |
|---|---|---|
| PCR-378-1ML | 1 ml x 100 μM | 108,90 € |
| PCR-378-10ML | 10 ml x 100 μM | 544,40 € |
| PCR-378-100ML | 100 ml x 100 μM | Ask for Quotation |
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Scientific citations for Jena Bioscience pcr-378
32 scientific citations and 1 citation images found by Bioz.
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Integration of gene expression profiling and triterpenic acids content reveals optimal harvesting seasons in Salvia rosmarinus
Scientific Reports, 2026-09-13 · DOI:10.1038/s41598-026-71628-6
.. using a Rotor-Gene Q thermocycler (QIAGEN, Germany) under optimized conditions specific to each gene.. The reaction volume was 10 μL, containing 5.0 μL
SYBR Green Fluorescent DNA Stain–low ROX Master Mix (Jena Bioscience ), 1.0 μL of each gene-specific forward and reverse primer, 2.0 μL cDNA, and 1.0 μL DEPC water.. Each cDNA sample was analyzed in triplicate.Each cDNA sample was analyzed in triplicate. -
Gut protist diversity in Eurasian beavers (Castor fiber) revealed by metabarcoding
International Journal for Parasitology: Parasites and Wildlife, 2026-08-03 · DOI:10.1016/j.ijppaw.2026.101270
The qPCR reaction mix contained 10 μl of 2x Q5 polymerase master mix (New England Biolabs, MA, United States), 1 μl of each forward and reverse primer mix (0.1 μM final concentration),
2 μl ofGreenFluorescent DNA Stain (1 μM final concentration) (Jena Biosciences , Germany), 5 μl of nuclease-free water, and 1 μl (1 ng) of genomic DNA. -
Engineering Paracoccus denitrificans PD1222 for Fusarium solani cutinase-mediated biodegradation of poly(butylene adipate-co-terephthalate).
Applied and Environmental Microbiology, 2026-06-18 · PMID:42313055
.. each primer set was calculated using standard curves from serial dilutions of the template.. The RT-qPCR reactions were carried out using the TaqPath DuraPlex 1-Step RT-qPCR Master Mix (Applied Biosystems, cat. no. A58666) and supplemented with Green-Fluo rescent DNA Stain (
Jena Bioscience , cat.no. PCR-378 ) for fluorescence detection.. All reactions followed the manufacturer’s recommendations.All reactions followed the manufacturer’s recommendations.
