DNA Intercalating dye for real-time qPCR fluorescence detection
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Form: liquid, orange, Supplied in 20 mM Tris-HCl pH 8.4, 0.1 mM EDTA and 0.01 % Tween-20
Concentration: 50x conc.
Spectroscopic Properties: λexc 500 nm (bound to DNA); λem 530 nm (bound to DNA)
EvaGreen® fluorescent DNA stain is supplied as 50x concentration.
Vortex EvaGreen® Fluorescent DNA Stain thoroughly prior to its use. Add 1 μl EvaGreen® Fluorescent DNA Stain for each 50 μl of the assay. Please note that the preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors.
To perform the EvaGreen-based assay simply select the optical setting for SYBR® Green on the detection instrument.
EvaGreen® Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR) and high-resolution DNA melting curve analysis (HRM). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
The high quantum yield, excellent stability and lowest inhibition toward PCR makes it the ideal fluorophore in real-time PCR applications and a superior replacement for the widely used SYBR® Green I dye.
EvaGreen® is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.