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ROX Reference Dye

Reference dye for fluorescence signal normalization

Cat. No. Amount Price (EUR) Buy / Note
PCR-351 1 ml (25 μM) 25,85 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
store dark

Shelf Life: 12 months

Form: liquid, pink, supplied in 20 mM Tris-HCl pH 8.4, 0.1 mM EDTA and 0.01 % Tween-20.

Concentration: 25 μM (50x conc.)

Spectroscopic Properties: λexc 576 nm; λem 601 nm;

Description:
ROX reference dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 50x concentration.

Preparation of the qPCR master mix:
Preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Vortex the ROX reference dye thoroughly before use. Add ROX reference dye to your PCR assay as indicated below.

final ROX
concentration
20 μl PCR assay50 μl PCR assay
low ROX assay
50 nM
0.04 μl0.1 μl
high ROX assay
500 nM
0.4 μl1.0 μl

Related products:

  • qPCR Core Kits
  • Dual labeled fluorescent probes
  • Custom primers