The JBS qPCR Multiplex Master Series has been specially developed and optimized for simultaneous real-time analysis of >4 target sequences in a single tube. The system overcomes multiplex limitations of conventional qPCR probe mixes combining with an above-average robustness for a multitude of known PCR inhibitors and an excellent sensitivity for amplification of lowest template amounts.
The 2x concentrated master mixes contain all reagents required for qPCR (except template, primers and labeled fluorescent probes) including a highly processive antibody-inhibited hot-start polymerase and ultra-pure dNTPs.
The mixes provide an extremely stringent automatic hot-start allowing reaction set-up and temporary storage at room temperature prior to PCR.
qPCR Multiplex Master Mixes are available in combination with low ROX and high ROX as reference dye for cycler-internal signal normalization. All mixes are recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes.