JBS qPCR GreenMaster Series allows quantitative real-time analysis of DNA samples based on EvaGreen™ fluorescent DNA stain. During PCR, EvaGreen™ intercalates into the amplified DNA. No synthesis of labeled probes is required, thus target DNA may rapidly be analyzed. Master Mixes contain all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a premixed 2x concentrated solution, including hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of initial denaturation to prevent extension of nonspecifically annealed primers and primer-dimer formation.
Both qPCR GreenMaster mixes with and without UNG are available in combination with low ROX and high ROX as reference dye for cycler-internal signal normalization. To facilitate pipetting into opaque plasticware a blue dyed version of the qPCR ProbesMaster Mixes is available.
EvaGreen™ Fluorescent DNA Stain
EvaGreen™ Fluorescent DNA Stain is developed for DNA analysis including real-time PCR (qPCR), high-resolution DNA melting curve analysis (HRM), routine DNA quantification and capillary gel electrophoresis. Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically.