JBS qPCR Core Kits are designed for quantitative real-time analysis of DNA samples based on dual labeled probes as well as EvaGreen™ fluorescent intercalator dye.
The high specificity and sensitivity of the Core Kits is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The included dNTP mix contains dUTP instead of dTTP and allows an UNG (Uracil-N-Glycosylase) treatment at the onset of thermal cycling to prevent carry-over contaminations of DNA from previous PCR reactions.