TaqMan® Detection & Multiplex

JBS qPCR Master Series allows quantitative real-time analysis of DNA samples based on detection of labeled DNA probes. Master Mixes contain all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a premixed 2x concentrated solution, including hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of initial denaturation to prevent extension of nonspecifically annealed primers and primer-dimer formation.

Key benefits

• Fast cycling-capable – 40 cycles in less than 25 min
• Flexible protocols – suitable for both standard and fast cycling workflows
• High sensitivity and precision across a broad dynamic range
• Optimized hot-start polymerase for specific amplification
• Compatible with dual-labeled probes, Molecular Beacons and FRET probes
• Suitable for multiplex applications
• Ready-to-use 2× master mix for convenient and reproducible reaction setup

Both qPCR ProbesMaster mixes with and without UNG (Uracil-N-Glycosylase) are available in combination with low ROX and high ROX as reference dye for cycler-internal signal normalization. To facilitate pipetting into opaque plasticware a blue dyed version of the qPCR ProbesMaster Mixes is available. Mixes are recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes.

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® TaqMan is a trademark of Roche Diagnostics GmbH