qPCR GreenMaster
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
qPCR GreenMaster is designed for quantitative real-time analysis of DNA samples. The mix contains all reagents required for qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. It is recommended for routine PCR applications, high throughput PCR or genotyping and provides an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds.
The mix is based on an optimized hot-start polymerase. Its activity is blocked by antibody at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The green-fluorescent DNA stain intercalates into the amplification product during the PCR process and allows the direct quantification of target DNA without the use of additional sequence-specific labeled probes (e.g. TaqMan® Probes).
The mix can also be combined with ROX reference dye (#PCR-351) in PCR instruments that are compatible with the evaluation of the ROX signal.
qPCR GreenMaster is also available as qPCR GreenMaster Lyophilisate, #PCR-173 in a freeze-dried (lyophilized) version that is pre-aliquoted in splittable 96-well plates, stable at ambient temperature, significantly reducing the risk of contamination, turnaround time and overall cost.
Green-fluorescent DNA stain
Green-fluorescent DNA stain is structurally similar to SYBR® GREEN and a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable, providing convenience during routine handling.
Green-fluorescent DNA stain is not recommended for high-resolution melting curve analysis (HRM).
To perform the assay, simply select the optical setting for SYBR® GREEN on the detection instrument.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, store dark
Storage at 4 °C for up to 3 months possible.
Shelf Life: 12 months
Form: liquid
Concentration: 2x conc.
Spectroscopic Properties: λexc 494 nm (bound to DNA), λem 521 nm (bound to DNA)
Content:
qPCR GreenMaster (red cap)
antibody-blocked hot start polymerase, dATP, dCTP, dGTP, dTTP, KCl, (NH4)2SO4, MgCl2, green-fluorescent DNA stain, additives and stabilizers
PCR-grade water
Preparation of the qPCR master mix:
The preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Prepare a master mix of all components except template as specified. A reaction volume of 20-50 μl is recommended for most real-time instruments. Prepare 13 volumes of master mix for 12 samples or a triple-set of 4 samples. Pipet with sterile filter tips and minimize the exposure of the master mix to light. Perform the setup in an area separate from DNA preparation or analysis. No-template controls should be included in all amplifications.
| component | 20 μl assay | 50 μl assay | final conc. |
|---|---|---|---|
| qPCR GreenMaster | 10 μl | 25 μl | 1x |
| primer forward (10 μM)1) | 0.6 μl | 1.5 μl | 300 nM |
| primer reverse (10 μM)1) | 0.6 μl | 1.5 μl | 300 nM |
| template DNA | x μl | x μl | - |
| PCR-grade water | fill up to 20 μl | fill up to 50 μl | - |
1) The optimal concentration of each primer may vary from 100 to 500 nM.
Dispensing the master mix:
Vortex the master mix thoroughly to assure homogeneity and dispense the mix into real-time PCR tubes or wells of the PCR plate.
Addition of template DNA:
Add the remaining x μl of sample/template DNA to each reaction vessel containing the master mix and cap or seal the tubes/plate. Do not exceed 500 ng DNA per reaction as final concentration. Tubes or plates should be centrifuged before cycling to remove possible bubbles.
Recommended cycling conditions:
| Initial denaturation and polymerase activation | 95 °C | 2 min | 1x |
| Denaturation | 95 °C | 15 sec | 35-45x |
| Annealing and elongation | 60-65 °C3) | 1 min4) | 35-45x |
3) The annealing temperature depends on the melting temperature of used primers.
4) The elongation time depends on the length of the amplicon. A time of 30 - 60 sec. for a fragment of up to 500 bp is recommended.
For optimal specificity and amplification an individual optimization of the recommended parameters, especially of the annealing temperature may be necessary for each new combination of template DNA and primer pair.
® SYBR is a trademark of Molecular Probes, Inc.
® TaqMan is a trademark of Roche Diagnostics GmbH
Related products:
- qPCR GreenMaster Lyophilisate, #PCR-173
- Dual-labeled DNA probes
- ROX reference dye, #PCR-351
- Thermolabile UNG, #PCR-353"
| Cat. No. | Amount | Price |
|---|---|---|
| PCR-372S | 2 x 1,25 ml (250 reactions x 20 μl) | 89,53 € |
| PCR-372L | 10 x 1,25 ml (1.250 reactions x 20 μl) | 358,05 € |
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Scientific citations for Jena Bioscience pcr-372
143 scientific citations and 12 citation images found by Bioz.
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The effect of toll like receptors in prognosis in patients undergoing radical cystectomy for bladder cancer.
Molecular Biology Reports, 2026-08-29 · PMID:42667340
Abstract: We aimed to evaluate the prognostic significance of TLR4 expression in bladder cancer, its association with genes related to EMT, and the potential regulatory role of selected microRNAs. Tumor and adjacent healthy bladder tissue samples obtained from 25 patients who underwent radical cystectomy for bladder cancer were analysed. The expression levels of TLR4, SPRR2A, SPRR1A, SPRR2B, ZEB2 and CDH1, in addition to miR-200c-3p and miR-146a-5p were measured using the RT-PCR method
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Agmatine diminishes pro-inflammatory response by modulating IL-1β and NF-κB expression in the prefrontal cortex and reverses behavioral impairments following chronic social isolation in rats.
Pharmacology, biochemistry, and behavior, 2026-07-10 · PMID:41786092
Abstract: Chronic social isolation (SI) is a significant psychosocial stressor associated with psychiatric disorders, including anxiety and depression, as well as cognitive decline. Emerging evidence suggests that neuroinflammation and oxidative stress in critical brain regions, such as prefrontal cortex (PFC), play a pivotal role in these conditions. Agmatine (AGM), an endogenous amine characterized by neuromodulatory properties, has exhibited anti-inflammatory, anxiolytic, and antidepressant effects
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Disrupting Pathogenicity in Foodborne Staphylococcus aureus : Biofilm Inhibition and Attenuation of Resistance and Virulence by Tunisian Aromatic Plant Essential Oils
Foods, 2026-07-02 · DOI:10.3390/foods15132361
.. subjected to quantitative real-time PCR using the Rotor-Gene Q MDx system (Qiagen, Hilden, Germany).. Each sample was analyzed in triplicate in a
20 μL reaction containing10 μL ofqPCR GreenMaster (Jena Bioscience ; Cat. No.PCR-372 L
