The JBS qPCR GreenMaster Series is designed for quantitative real-time analysis of DNA samples. The mixes contain all reagents required for qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. The mixes are recommended for routine PCR applications, high throughput PCR or genotyping and provide an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds.
The green-fluorescent DNA stain intercalates into the amplification product during the PCR process and allows the direct quantification of target DNA without the need of sequence-specific labeled probes.
Green-fluorescent DNA stain is structurally similar to SYBR® GREEN and a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable, providing convenience during routine handling. Green-fluorescent DNA stain not recommended for high-resolution melting curve analysis (HRM).
UNG (Uracil-N-Glycosylase) eliminates carry-over contamination of DNA from previous PCR reactions. An UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation.
® SYBR is a trademark of Molecular Probes, Inc.
