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Red Load Taq Master Lyophilisate

Lyophilized Taq Master Mix containing red gel loading dye

Cat. No. Amount Price (EUR) Buy / Note
PCR-151S-8TS 12 8-tube strips (96 reactions) 93,06 Add to Basket/Quote Add to Notepad
PCR-151L-8TS 60 8-tube strips (480 reactions) 360,00 Add to Basket/Quote Add to Notepad
PCR-151S-FTP 2 96-well plates (flat top-w/o skirt) (192 reactions) 135,00 Add to Basket/Quote Add to Notepad
PCR-151L-FTP 10 96-well plates (flat top-w/o skirt) (960 reactions) 540,00 Add to Basket/Quote Add to Notepad
PCR-151S-HSP 2 96-well plates (half skirt) (192 reactions) 135,00 Add to Basket/Quote Add to Notepad
PCR-151L-HSP 10 96-well plates (half skirt) (960 reactions) 540,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at ambient temperature
Store in an aluminium-coated bag or on a dry place.
Lyophilisates may hydrate at humidity levels >70 % when sealing is opened.

Shelf Life: 12 months

Form: lyophilized

Description:
Red Load Taq Master Lyophilisate is delivered in PCR reaction tube strips or 96-well plates preloaded with a complete master mix in a dry, room temperature stable format. The lyophilisate combines highest performance with convenience of use and stability. There is no need for freezing, thawing or pipetting on ice. The few remaining pipetting steps minimize the risk of errors or contaminations.
Each vial contains polymerase, dNTPs and reaction buffer with MgCl2 required for a 20 μl PCR assay. The Red Load Taq Master Lyophilisate contains additionally an inherent red dye allowing the direct loading of the PCR reaction product onto the gel.
To perform PCR, fill up the vials with a premix of primers and PCR-grade water and add DNA template. If necessary, centrifuge to remove bubbles, vortex the vials to assure homogeneity and start cycling.

Content:
Red Load Taq Master Lyophilisate
Preloaded lyophilisates of Taq DNA polymerase, dATP, dCTP, dGTP, dTTP, Reaction Buffer, MgCl2, red gel loading dye and stabilizers.

PCR grade water

Recommended 20 μl PCR assay:

forward Primer0.2 - 1 μM (0.4 - 2 μl / 10 μM)
reverse Primer0.2 - 1 μM (0.4 - 2 μl / 10 μM)
template DNA1 - 50 ng
PCR-grade waterfill up to 20 μl

Recommended cycling conditions:

Initial
denaturation
94 °C2 min1x
Denaturation94 °C30 sec30x
Annealing1)50 - 68 °C30 sec30x
Elongation2)72 °C30 sec - 3 min30x
Final
elongation
72 °C2 min1x
1) The annealing temperature depends on the melting temperature of the primers used.
2) The elongation time depends on the length of the fragments to be amplified. 1 min/kb elongation time is recommended.


For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new primer-template pair.