Detection of poly-histidine tagged proteins, ready-to-use solution
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months (3 months upon opening)
His Detect Solution is a ready-to-use reagent for detection of poly-histidine tagged proteins in blot assays. The reagent is equally suitable for Western Blot, Dot or Slot Blot assays. The detection reagent is made from an optimized anti-histidine antibody gold nanoparticle conjugate and is reactive against C- and anti-N terminal tags. The protein of interest is specifically stained in pink directly on the blot. Instant detection of poly-histidine tagged proteins on nitrocellulose membrane with His Detect is easy and does not require relevant hands on time. No blocking, Washing or successive antibody incubation time needed. A detection limit of 0.1 pmol can be achieved with this highly sensitive detection reagent. This is in the range of conventional ECL detection.
30 ml His Detection reagent
Additional Materials Required:
There is no detection equipment required. The detection signal is visible by the human eye and may be quantified by spectroscopic or photo-technical equipment. The intensity of the band is proportional to the amount of protein present, thus quantification can be easily achieved with standard spotted next to the sample.