Detection of poly-histidine tagged proteins, ready-to-use solution
Cat. No. | Amount | Price (EUR) | Buy / Note |
---|---|---|---|
PS-110 | 30 ml (up to 9 reactions) | 220,40 | Add to Basket/Quote Add to Notepad |
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months (3 months upon opening)
Description:
His Detect Solution is a ready-to-use reagent for detection of poly-histidine tagged proteins in blot assays. The reagent is equally suitable for Western Blot, Dot or Slot Blot assays. The detection reagent is made from an optimized anti-histidine antibody gold nanoparticle conjugate and is reactive against C- and anti-N terminal tags. The protein of interest is specifically stained in pink directly on the blot. Instant detection of poly-histidine tagged proteins on nitrocellulose membrane with His Detect is easy and does not require relevant hands on time. No blocking, Washing or successive antibody incubation time needed. A detection limit of 0.1 pmol can be achieved with this highly sensitive detection reagent. This is in the range of conventional ECL detection.
Content:
30 ml His Detection reagent
Additional Materials Required:
Preparation Procedure:
There is no detection equipment required. The detection signal is visible by the human eye and may be quantified by spectroscopic or photo-technical equipment. The intensity of the band is proportional to the amount of protein present, thus quantification can be easily achieved with standard spotted next to the sample.