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RNase A (DNase free)

Endoribonuclease

Bovine pancreas

Cat. No. Amount Price (EUR) Buy / Note
EN-179S 100 mg 93,60 Add to Basket/Quote Add to Notepad
EN-179L 5 x 100 mg 374,60 Add to Basket/Quote Add to Notepad

For in vitro use only!

Unit Definition: 1 unit corresponds to the amount of enzyme which hydrolyses RNA at a rate constant k = 1 at 25°C and pH 5.0 (Kunitz-units).

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Molecular Weight: 13.7 kDa

CAS#: 9001-99-4

EC number: 232-646-6

Form: dry powder

Activity: ≥70 kunits/mg

Applications:
RNase protection assays
Remove unspecifically bound RNA
Analysis of RNA sequences
Hydrolyse RNA contained in protein samples
Purification of DNA

Description:
RNase A specifically cleaves at the 3'-side of pyrimidine (uracil or cytosine) phosphate bonds

optimum reaction temperature: 60 °C (working range 15-70 °C)

optimum pH value: 7.5 (working range 6-10)

Reconstitution: RNase A should be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl pH 7.5, 15 mM NaCl.

Product Citations:
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