bacteriophage T4, recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 27 kDa
Accession number: AAD42414
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 100 mM Tris-HCl pH 7.5, 10 mM MgCl2 and 50 % glycerol)
Activity: One unit of enzyme catalyses the phosphorylation of 1 nmol dGMP to dGDP per minute at 37°C. Specific activity: 100 u/μg.
NMP kinases catalyse the phosphorylation of nucleotide monophosphates resulting in the corresponding nucleotide diphosphates by using ATP as a phosphate donor. T4 dNMP kinase is the only member of the family of NMP kinases that recognizes three structurally dissimilar nucleotides: dGMP, dTMP and 5-hydroxymethyl-dCMP (5-OH-dCMP).
Activity Assay: 1 μg T4 dNMP kinase, 2 mM ATP and 2 mM dGMP in 100 mM Tris-HCl pH 7.5, 10 mM MgCl2.
Teplyakov et al. (1996) Crystal structure of bacteriophage T4 deoxynucleotide kinase with its substrates dGMP and ATP. EMBO J. 15:3487.
Brush et al. (1993) Chemical modification of bacteriophage T4 deoxynucleotide kinase. Evidence of a single catalytic region. J. Biol. Chem. 268:1603.
Brush et al. (1990) Bacteriophage T4 deoxynucleotide kinase: gene cloning and enzyme purification. J. Bacteriol. 172:2935.