recombinant, Pichia pastoris
For in vitro use only!
Unit Definition: One unit of rSAP releases 1 μmol phosphate/min from 4-nitrophenyl phosphate in 0.1 M glycine-NaOH pH 10.4, 1 mM MgCl2, 1 mM ZnCl2 and 6 mM 4-nitrophenyl phosphate.
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE)
Concentration: 1 unit/μl
> 2000 units/mg
Optimum working range is between pH 7-9. rSAP is active in most restriction- and PCR-buffers. Mg2+ (>1 mM) is required for activity.
Recombinant Shrimp Alkaline Phosphatase is a multipurpose alkaline phosphatase that can be fully inactivated by a short heat treatment. This property simplifies most workflows involving alkaline phosphatase treatment. The recombinant form of SAP replaces the native form that has been established on the market for several years. rSAP has all the properties of the well proven SAP, but with additional benefits. rSAP is far more stable at ambient temperature, it is of high, consistent purity, and is available in large batches at high concentration.
rSAP is completely inactivated by a 5 min incubation at 65 °C.
Absence of contaminants:
Tested for the absence of exonucleases, endonucleases and Rnases.
Chakraborty et al. (2011) Active site detection by spatial conformity and electrostatic analysis-unravelling a proteolytic function in shrimp alkaline phosphatase. PLoS One. 6: e28470.
de Bakcer et al. (2002) The 1.9 A crystal structure of heat-labile shrimp alkaline phosphatase. J Mol Biol. 18:1265.
Nilsen et al. (2001) Thermolabile alkaline phosphatase from Northern shrimp (Pandalus borealis): protein and cDNA sequence analyses. Comp Biochem Physiol B Biochem Mol Biol. 129:853.