For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Direct WGA Kit is a complete system for whole genome amplification from various tissues or samples directly without DNA purification processes. Very little amount of samples, several miligram or microliter volume, are required for the direct WGA. About 10 μg DNA products could be obtained in a standard reaction. The enzyme mix and buffer system are designed to tolerate against most amplification inhibitors found in crude samples. Phi29 DNA polymerase, the major polymerization enzyme of this kit, isothermally amplifies the genomic DNAs included in the samples with multiple displacement mechanism. Phi29 DNA polymerase could produce DNA strand up to 70 kb long with high fidelity. All required components including enzymes, buffers, dNTPs, random primers, and sample pretreatment reagents are supplied in this kit. The amplified DNA products could be applied for successive PCR, genotyping, and library construction.
|1 M DTT||100 μl||500 μl|
|PBS Buffer||20 μl||100 μl|
|DB||1.0 ml||5 x 1.0 ml|
|NB||40 μl||200 μl|
|Primer Mix||20 μl||100 μl|
|Enzyme Mix||20 μl||100 μl|
|Reaction Buffer||240 μl||1.2 ml|
|dNTP Mix (each 10 mM)||40 μl||200 μl|
1. Preparation of DM Buffer
2. Sample Preparation
for Blood Samples
for Animal tissue
for Plant Leaves or Seeds
3. Add followings and mix well to make final 20 μl reaction mixture
|component||20 μl assay|
|Reaction Buffer||12 μl|
|Primer Mix||1 μl|
|Enzyme Mix||1 μl|
Component DB contains dangerous substance
H302 Harmful if swallowed.
H314 Causes severe skin burns and eye damage.
H412 Harmful to aquatic life with long lasting effects.
P260 Do not breathe dust/fume/gas/mist/vapours/spray.
P301 + P312 IF SWALLOWED: Call a POISON CENTER/doctor/.../ if you feel unwell.
P301 + P330 + P331 IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water [or shower].
P304 + P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
For further information see Material Safety Data Sheet.