» Sign in / Register

Phi29 DNA Polymerase

Bacillus subtilis phage phi29, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PCR-381S 200 units 49,50 Add to Basket/Quote Add to Notepad
PCR-381L 1.000 units 198,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Unit Definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74 °C.

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid

Concentration: 10 units/μl

Applications:

  • Rolling-circle amplification (RCA)
  • Multiple displacement amplification (MDA)
  • Whole genome amplification (WGA)
  • Preparation of DNA template for sequencing

Description:
Phi29 DNA polymerase is a recombinant protein purified from E. coli cloned the gene encoding the DNA polymerase from Phi29 phage. Phi29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage Phi29 and possesses the highest processivity and strand-displacement activity among the known DNA polymerase.
Phi29 DNA polymerase contains a 3'→5' exonuclease activity that enables proofreading capability.

Content:
Phi29 DNA Polymerase (brown cap)
10 units/μl Phi29 DNA Polymerase in storage buffer
(25 mM NaH2PO4 pH 7.0, 150 mM NaCl, 125 mM Imidazole, 50 % Glycerol, 2.5 mM 2-Mercaptoethanol)

Reaction Buffer (purple cap)
10x conc. complete reaction buffer containing 500 mM Tris-HCl pH 7.5, 100 mM MgCl2, 100 mM (NH2)4SO4 and 40 mM DTT

componentPCR-381SPCR-381L
Phi29 DNA Polymerase20 μl100 μl
Reaction Buffer100 μl500 μl


Recommended Assay Set-Up

componentCat. No.stock conc.final conc.1 assay
@ 20 μl
PCR-grade WaterPCR-258fill up to 20 μl
Reaction BufferPCR-381
(purple cap)
10x1x2 μl
dNTP MixNU-100610 mM125 μM0.25 μl
Random HexamersPM-301100 μM5 μM1 μl
Target DNA1 μl
Phi29 DNA PolymerasePCR-381
(brown cap)
10 units/μl5-10 units/assay5-10 units

Incubation:
Incubate at 30 °C.

Inactivation:
Heat the mixture to 65 °C for 15 min.

Related products: