Guanosine 5'-triphosphate, Disodium salt trihydrate
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H16N5O14P3 (free acid)
Molecular Weight: 523.18 g/mol (free acid)
Purity: ≥ 90 % (HPLC)
Spectroscopic Properties: λmax 252 nm, ε 14.2 L mmol-1 cm-1 (Tris-HCl pH 7.0)
Assembly of ribosomal units
Microdomain formation by small GTPases
Antiviral activity of large GTPases (dynamin superfamily)
Regulation of exocytosis by Rho GTPases
Mechanism of hydrolysis by ADP-ribosylation factors
Guanylate binding proteins
Quality Control Specifications: in vitro transcription (T7 RNA polymerase): visible RNA fragments after 5 min incubation; DNases, RNases, Nicking Activity: not detectable; Proteases: not detectable
 Blombach et al. (2011) Assembling the archeal ribosome: roles for transition factor-related GTPases. Biochemical Society Transactions 39:45.
 Stuermer (2011) Microdomain-forming proteins and the role of the reggies/flottilins during axon regeneration in zebrafish. Biochimica Biophysica Acta, Molecular Basis of Disease 1812:415.
 Haller et al. (2011) Human MxA protein: An Interferon-induced Dynamin-like GTPase with broad antiviral activity. J. Interferon and Cytokine Research 31:79.
 Stephane et al. (2011) Rho GTPases and exocytosis: what are the molecular links? Seminars in Cell and Developmental Biology 22:27.
 East et al. (2011) Models for the function of Arf GAPs. Seminars in Cell and Developmentan Biology 22:3.
 Vestal et al. (2011) The guanylate binding proteins: Emerging insights into the biochemical properties and functions of this family of large interferon-induced guanosine triphosphatase. J. Interferon and Cytokine Research 31:89.
 Younghoon et al. (2011) Septin structure and function in yeast and beyond. Trends in Cell Biology 21:141.
Drummond et al. (2011) Reconstitution and Organization of Escherichia coli Proto-ring Elements (FtsZ and FtsA) inside Giant Unilamellar Vesicles Obtained from Bacterial Inner Membranes. Methods Mol. Biol. 777 :29.
Katsuki et al. (2011) Preparation of dual-color polarity-marked fluorescent microtubule seeds. Methods Mol. Biol. 777:117.
Ramachandran et al. (2009) Membrane Insertion of the Pleckstrin Homology Domain Variable Loop 1 Is Critical for Dynamin-catalyzed Vesicle Scission. Molecular Biology of the Cell 20 (22):4630.