RafRBD (c-Raf)
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
Raf is a member of the serine/threonine proteine kinase family involved in regulation of cell growth and differentiation and is the most important effector of Ras. Full-length Raf is composed of three conserved regions responsible for interaction with Ras, for phosphorylation and for catalytic activity. RafRBD (Ras binding domain, amino acids 50-132) mediates interaction with membrane-anchored Ras necessary for activation of the kinase activity of Raf.
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 9.4 kDa (50 - 132 amino acids)
Accession number: X03484 / P04049
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 32 mM Tris-HCl pH 7.6, 200 mM NaCl, 5 mM MgCl2 and 5 mM DTT)
Selected References:
Eing et al. (2002) Quantification of the Raf-C1 interaction with solidsupported bilayers. ChemBioChem 3:190.
Emerson et al. (1996) Structure of Ras-binding domain of c-Raf-1 as determined by NMR spectroscopy and identification of the region that interacts with Ras. Drug Des. Discov. 13:83.
Nasser et al. (1995) The 2.2 crystal structure of the Ras-binding domain of the serine/threonine kinase c-Raf1 in complex with Rap1A and a GTP analogue. Nature 375:554.
Bahk et al. (2008) A Cross-talk between oncogenic Ras and tumor suppressor PTEN through FAK Tyr861 phosphorylation in NIH/3T3 mouse embryonic fibroblasts. Biochemical and Biophysical Research Communications 377 (4):1199-1204.
At this point, external content from bioz.com is embedded. When it loads, data (e.g. your IP address) is transmitted to the provider.
Scientific citations for Jena Bioscience pr-305
3 scientific citations and 0 citation images found by Bioz.
-
Mega-High-Throughput Screening Platform for the Discovery of Biologically Relevant Sequence-Defined Non-Natural Polymers
ACS Central Science, 2022-01-11 · DOI:10.1021/acscentsci.1c01041
.. Inc. K-Ras4B G12V (K-Ras; catalog number: CS-RS04) was purchased from Cytoskeleton Inc. (Denver, Colorado)..
Raf-RBD (Raf; catalog number: PR-305) was purchased fromJena Bioscience (Jena, Germany).. Recombinant human interleukin-6 protein (IL-6; catalog number: IL6-12H) and recombinant human interleukin-6 receptor (IL-6R; .. -
What makes Ras an efficient molecular switch: a computational, biophysical, and structural study of Ras-GDP interactions with mutants of Raf.
Journal of Molecular Biology, 2010-09-03 · PMID:20361980
Abstract: Ras is a small GTP-binding protein that is an essential molecular switch for a wide variety of signaling pathways including the control of cell proliferation, cell cycle progression and apoptosis. In the GTP-bound state, Ras can interact with its effectors, triggering various signaling cascades in the cell. In the GDP-bound state, Ras looses its ability to bind to known effectors
-
A cross-talk between oncogenic Ras and tumor suppressor PTEN through FAK Tyr861 phosphorylation in NIH/3T3 mouse embryonic fibroblasts.
Biochemical and biophysical research communications, 2008-12-16 · PMID:19000654
Abstract: Although Ras is a potent oncogene, its tumorigenicity depends on cellular context and cooperative events. Tumor suppressor PTEN is the most important negative regulator of the cell-survival signaling pathway initiated by phosphoinositide 3-OH kinase. Previously, we established various NIH/3T3 cells expressing H-Ras mutant proteins
