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Crystal Taq Master (2x)

Master mix for routine PCR

Ready-to-Use Mixes for PCR

Cat. No. Amount Price (EUR) Buy / Note
PCR-166S 4 x 1,25 ml (2x conc.) 77,81 Add to Basket/Quote Add to Notepad
PCR-166L 20 x 1.25 ml (2x conc.) 311,26 Add to Basket/Quote Add to Notepad
PCR-166XL 125 ml (2x conc.) 1.149,98 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 3 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid

Concentration: 2x conc.

Description:
Crystal PCR Master is a 2 x conc. ready-to-use master mix recommended for routine PCR applications (up to 4 kb fragment length), high throughput PCR or genotyping.
It contains all reagents required for PCR (except template and primer) in a well-balanced ratio to ensure high specificity and minimal by-product formation in almost all PCR applications without the need of additional optimization steps.
The mix guarantees robust and reliable amplification results with a minimum of pipetting steps, saves time and reduces the risk of contaminations.
The total PCR assay volume is freely adaptable to individual protocols or the requirements of automated pipetting systems.

Content:

Cat.No.Master MixPCR-grade waterAssays x 50 μl
PCR-166S4 x 1.25 ml6 ml200
PCR-166L20 x 1.25 ml2 x 12.5 ml1000
PCR-166XL125 ml125 ml5000

2 x concentrated PCR master mix containing Taq polymerase, nucleotides (dATP, dCTP, dGTP, dTTP), KCl, (NH4)2SO4, MgCl2, density reagent, enhancing and stabilizing additives.

Recommended PCR assay:
Before starting, vortex the master mix thoroughly to assure homogeneity.

componentstock. conc.20 μl
assay
50 μl
assay
final conc.
Crystal PCR Master2x10 μl25μl1x
Primer Mix
or each primer
10 μM each primer0.4-0.8 μl1-2 μl200-400 nM each primer
Template/ sample DNA < 10 ng < 20 ng
PCR-grade water fill up to 20 μlfill up to 50 μl

Recommended cycling conditions:
Before cycling, vortex PCR tubes or plates to assure homogeneity and centrifuge briefly to remove bubbles.

Initial
denaturation
95 °C2 min1x
Denaturation
Annealing1)
Elongation2)
95 °C
50 - 68 °C
72 °C
10 - 20 sec
10 - 20 sec
20 sec - 4 min

25 - 30x

1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kbp is recommended.