Red master mix for routine PCR and direct gel loading
Ready-to-Use Mixes for PCR
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term storage (up to 3 month) at 4 °C possible.
Shelf Life: 12 months
Concentration: 2x conc.
Ruby PCR Master is a 2 x conc. ready-to-use master mix recommended for routine PCR applications (up to 4 kb fragment length), high throughput PCR or genotyping.
It contains all reagents required for PCR (except template and primer) in a well-balanced ratio to ensure high specificity and minimal by-product formation in almost all PCR applications without the need of additional optimization steps.
The mix contains gel loading buffer and an inherent red dye allowing the direct loading of the PCR product into the gel.
The red dye allows an easy visual control during PCR set-up and in combination with the density reagent the direct loading of the reaction product into the gel.
The mix guarantees robust and reliable amplification results with a minimum of pipetting steps, saves time and reduces the risk of contaminations.
The total PCR assay volume is freely adaptable to individual protocols or the requirements of automated pipetting systems.
|Cat.No.||Master Mix||PCR-grade water||Assays x 50 μl|
|PCR-164S||4 x 1.25 ml||6 ml||200|
|PCR-164L||20 x 1.25 ml||2 x 12.5 ml||1000|
|PCR-164XL||125 ml||125 ml||5000|
2 x concentrated PCR master mix containing Taq polymerase, nucleotides (dATP, dCTP, dGTP, dTTP), KCl, (NH4)2SO4, MgCl2, red dye, density reagent, enhancing and stabilizing additives.
Recommended PCR assay:
Before starting, vortex the master mix thoroughly to assure homogeneity.
|component||stock. conc.||20 μl|
|Ruby PCR Master||2x||10 μl||25μl||1x|
or each primer
|10 μM each primer||0.4-0.8 μl||1-2 μl||200-400 nM each primer|
|Template/ sample DNA||< 10 ng||< 20 ng|
|PCR-grade water||fill up to 20 μl||fill up to 50 μl|
Recommended cycling conditions:
Before cycling, vortex PCR tubes or plates to assure homogeneity and centrifuge briefly to remove bubbles.
|95 °C||2 min||1x|
50 - 68 °C
|10 - 20 sec|
10 - 20 sec
20 sec - 4 min
25 - 30x