lyophilised Master Mix for direct PCR amplification from blood, animal and plant tissue
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Store in an aluminium-coated bag or on a dry place.
Lyophilisates may hydrate at humidity levels >70 % when sealing is opened.
Shelf Life: 12 months
Direct PCR Lyophilisate is delivered in PCR reaction tube strips or 96-well plates preloaded with a complete master mix in a dry, room temperature stable format. The lyophilisate combines highest performance with convenience of use and stability. There is no need for freezing, thawing or pipetting on ice. The few remaining pipetting steps minimize the risk of errors or contaminations.
To perform PCR, fill up the vials with primers, Additives Mix and PCR-grade water and add DNA template. If necessary, centrifuge to remove bubbles, vortex the vials to assure homogeneity and start cycling.
The lyophilisate is designed for PCR amplification directly from whole blood, animal tissues and plant tissues without the need of prior DNA purification processes.
The robust enzyme in combination with a specially optimized unique buffer system resists various PCR inhibitors of blood and tissue samples.
The enzyme lacks a 3'→5' exonuclease activity making this kit an ideal choice for allele specific PCR which is routinely used for various genotyping applications.
Direct PCR Lyophilisate
Preloaded lyophilisates of Direct PCR Mastermix containing Hot Start Taq polymerase, nucleotides, optimized reaction buffer, stabilizers
Additives Mix (purple cap)
2 x conc.
DNA Extraction Buffer (yellow cap)
10 x conc.
PCR grade water (white cap)
Whole blood or saliva
Animal or Plant tissue
Recommended PCR assay:
The preparation of a primer mix is recommended to minimize pipetting errors.
|Comp.||cap||stock conc.||final conc.||20 μl assay|
|Direct PCR Lyophilisate||1 x||1 tube|
|Additives Mix||purple||2 x||1 x||10 μl|
|forward Primer1)||10 μM||400 nM||0.8 μl|
|reverse Primer1)||10 μM||400 nM||0.8 μl|
|Sample Preparation||1-2 μl|
|PCR-grade water||white||fill up to 20 μl|
|95 °C||5 min||1x|
|Denaturation||95 °C||20 sec||35-40x|
|Annealing1)||45-68 °C||30 sec||35-40x|
|Elongation2)||72 °C||30 sec - 4 min||35-40x|
|Final elongation||72 °C||2 min||1x|