Amplification of a beta-actin gene fragment from human genomic DNA
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| PCR-253 | 500 reactions | 54,30 | Add to Basket/Quote Add to Notepad |
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, aliquot genomic DNA
Short term storage (up to 1 week) at 4 °C possible.
Shelf Life: 12 months
Form: liquid
Description:
PCR Control Kit is designed as tool to confirm or compare the quality and functionality of Taq DNA polymerases. The kit contains a forward and reverse primer-pair designed to amplify a 295 bp long fragment of the beta-actin gene from human genomic DNA.
Content:
beta-actin Primer Mix (white cap)
500 μl, 15 μM each
Human genomic DNA (purple cap)
500 μl, 10 ng/μl
Recommended 50 μl PCR assay:
| component | stock conc. | final conc. | amount |
| Reaction Buffer complete | 10x | 1x | 5 μl |
| dNTP Mix | 10 mM | 200 μM | 1 μl |
| beta-actin Primer Mix | 15 μM | 300 nM | 1 μl |
| Human genomic DNA | 10 ng/μl | 1-10 ng/assay | 0.1 - 1 μl |
| Taq Polymerase | 5 units/μl | 1.25 units/assay | 0.25 μl |
| PCR-grade water | - | - | fill up to 50 μl |
| initial denaturation | 95 °C | 2 min | 1x |
| denaturation | 95 °C | 10 sec | 30-35x |
| annealing | 59 °C | 20 sec | 30-35x |
| elongation | 72 °C | 30 sec | 30-35x |
| final elongation | 72 °C | 2 min | 1x |
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