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PCR Demo Kit - DNA Fragments

PCR Kit for amplification of 100 / 200 / 500 / 1,000 bp DNA fragments

Cat. No. Amount Price (EUR) Buy / Note
PCR-265 4 x 50 reactions 135,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid

Concentration: 10x conc.

Applications:
Taq Reaction Buffer complete (green cap)
200 mM Tris-HCl (pH 8.5 at 25 °C), 500 mM KCl and 15 mM MgCl2.
Taq Reaction Buffer without MgCl2 (blue cap)
200 mM Tris-HCl (pH 8.5 at 25 °C) and 500 mM KCl.
High Yield Buffer complete (green cap)
600 mM Tris-HCl (pH 8.8 at 25°C), 150 mM (NH4)2SO4, 20 mM MgCl2, 0.05 % Tween-20 and 0.05 % Nonidet P-40.
High Yield Buffer without MgCl2 (blue cap)
600 mM Tris-HCl (pH 8.8 at 25°C), 150 mM (NH4)2SO4, 0.05 % Tween-20 and 0.05 % Nonidet P-40.
Hot Start Buffer complete (green cap)
200 mM Tris-HCl (pH 8.5 at 25°C), 500 mM KCl and 15 mM MgCl2.
Hot Start Buffer without MgCl2 (blue cap)
200 mM Tris-HCl (pH 8.5 at 25°C) and 500 mM KCl.
MgCl2 Stock Solution (yellow cap)
25 mM MgCl2

Description:
PCR Reaction Buffers are ultrapure and sterile 10 x conc. reaction buffer for standard and routine PCR applications. MgCl2 stock solution is recommended for individual optimization of for PCR assays.

Optimization of MgCl2 concentration:
A concentration of 1.5 mM Mg2+ is recommended for most standard PCR applications. For an individual optimization use the reaction buffer without MgCl2 and add MgCl2 stock solution as shown in the table below.
20 μl PCR assay

final MgCl2 conc.1 mM1.5 mM2 mM3 mM
Reaction Buffer without MgCl2 - 10x2 μl2 μl2 μl2 μl
MgCl2 Stock Solution - 25 mM0.8 μl1.2 μl1.6 μl2.4 μl

50 μl PCR assay
2 conc.||1 mM||1.5 mM||2 mM||3 mM
Reaction Buffer without MgCl2 - 10x||10 μl||10 μl||10 μl||10 μl
MgCl2 Stock Solution - 25 mM||2 μl||3 μl||4 μl||6 μl

Availability Restriction: Exclusively distributed in Japan by Greiner BioOne