Positive control templates for PCR
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, aliquoting of DNA samples is recommended
Shelf Life: 12 months
Molecular Weight: 31.5 x 106 Dalton
Form: liquid (Supplied in 10 mM Tris-HCl pH 7.4 and 1 mM EDTA)
Concentration: 100 ng/μl
Lambda DNA is recommended as template in positive control PCRs, as substrate in restriction enzymes research and for testing of restriction endonucleases activity. The double stranded DNA is isolated from bacteriophage lambda (cl857 ind1 Sam7). Double stranded DNA with 48,502 base pairs.
The DNA is isolated from the purified phage by phenol/chloroform extraction.
Gel analysis for purity, EcoRI and HindIII fragmentation patterns.
Daniels et al. (1983) Appendix II: Complete annotated lambda sequence, R. W. Hendrix, J. W. Roberts, F. W.Stahl, and R. A. Weisberg, Eds. (Cold Spring Harbor Laboratory, Cold Spring Harbor 519 [LAMBDA II].
Daniels et al. (1983) Appendix I: A molecular map of coliphage lambda, R. W. Hendrix, J. W. Roberts, F. W.Stahl, and R. A. Weisberg, Eds. (Cold Spring Harbor Laboratory, Cold Spring Harbor 469. [LAMBDA II].
Sanger et al. (1982) Nucleotide sequence of bacteriophage lambda DNA. J. Mol. Biol. 162:729.