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Hot Start Taq Polymerase

Hot Start PCR technique reduces non-specific amplifications and offers a convenient reaction set-up at room temperature. The enzymatic activity of hot start polymerase is blocked by an aptamer or antibody at ambient temperature and switched on automatically during the increased temperature of the initial annealing step. The polymerase is recommended for routine PCR applications, high throughput PCR or genotyping and provides an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds.

Enzyme Efficiency /
Yield
Specificity Fidelity /
Error rate
Application
Taq Polymerase
Taq Pol (PCR-211)
++ ++ 10-5 Standard PCR / optimized for minimal by-product formation
Routine and plate based PCR, automated pipetting
Hot Start Taq Polymerase
Hot Start Pol Apta+ (PCR-212)
++ +++ 10-5 High specificity PCR / high sensitivity PCR
High throughput PCR
Hot Start Pol Ab+ (PCR-213) ++ ++++ 10-5 High specificity PCR / high sensitivity PCR
Diagnostic PCR
High Fidelity Polymerase
Taq HiFi Pol (PCR-204)
+++ ++ 2 x 10-6 High fidelity PCR / long range PCR (> 30 kb)
Amplification of GC-rich / difficult templates
Pfu-X Pol (PCR-207) +++ +++ 2 x 10-7 High speed amplification with highest fidelity
Amplification of difficult and long templates
Ultra DNA Pol (PCR-391) +++ +++ n/a Robust and fast PCR amplification of templates from difficult matrices