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Ultra DNA Polymerase

Robust and fast DNA Polymerase with enhanced fidelity

also known as Phusion High-Fidelity DNA Polymerase

Cat. No. Amount Price (EUR) Buy / Note
PCR-391L 500 units 378,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Unit Definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 70 °C.

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid

Concentration: 2.0 units/μl

Description:
Ultra DNA Polymerase is a genetically optimized DNA polymerase for robust, fast and accurate amplification, even with difficult or GC-rich DNA templates. The polymerase is based on Pfu with a fused DNA binding domain.
The polymerase is tolerant against various inhibitors allowing stable amplification with minimized assay optimization.
The enhanced processivity guarantees highly efficient amplification and makes the enzyme the ideal choice for routine applications in analytical or diagnostic assays, cloning and PCR with long or difficult templates.
With a 2x increased extension rate and a 10x increased fidelity compared to Taq, Ultra DNA Polymerase generates improved product yields at high speed without compromising accuracy.

Content:
Ultra DNA Polymerase
2.0 units/μl High Fidelity Polymerase in 20 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5 % Tween-20, 0.5 % Nonidet P-40, 50% (v/v) Glycerol, pH 8.0 (25 °C) and 0.2 mg/ml BSA

Ultra DNA Buffer
5x conc.

PCR Reaction Setup
The PCR procedure below shows appropriate volumes for a single 50 μl reaction. For multiple reactions, prepare a master mix of components common to all and then dispense appropriate volumes into each PCR reaction tube prior to adding template DNA and primers.
Thaw, mix, and briefly centrifuge each component before use.
Add the following components to a microcentrifuge tube:


Recommended 50 μl PCR assay:

comp.stock conc.final conc.1 assay @ 20 μl1 assay @ 50 μl
PCR-grade Waterfill up to 20 μlfill up to 50 μl
Ultra DNA Buffer5x1x4 μl10 μl
dNTP Mix / 10 mM #NU-100610 mM200 μM0.4 μl1 μl
Ultra DNA Polymerase2 units/μl0.025 units/μl0.2 μl0.5 μl
primer mix or each primer10 μM each primer200 - 400 nM each primer0.4 - 0.8 μl1 - 2 μl
template
/sample DNA
< 10 ng DNA< 20 ng DNA

Mix and briefly centrifuge the components.

Recommended cycling conditions:

initial
denaturation
98 °C30 sec1x
denaturation
annealing1)
elongation2)
98 °C
45-68 °C
68-72 °C
5-10 sec
10-30 sec
15-30 sec/kb
25-35x
final
elongation
72 °C5-10 min1x

1)The annealing temperature depends on the melting temperature of the primers used.
2)For fragments higher than 7 kbp use 68 °C

For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new template DNA and/or primer pair.

Related products:

  • Ready-to-Use Mixes / direct gel loading
  • Ready-to-Use Mixes
  • Thermophilic Polymerases
  • Deoxynucleotides (dNTPs)
  • Supplements
  • Primers and Oligonucleotides
  • DNA Ladders