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High Fidelity Polymerase

High Fidelity Polymerase is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.

Pfu-X Polymerase is the ideal choice for applications where efficient amplification of DNA with highest fidelity is required. The enzyme is an engineered Pfu DNA polymerase showing a higher accuracy and increased processivity, resulting in shorter elongation times.

Enzyme Efficiency /
Specificity Fidelity /
Error rate
Taq Polymerase
Taq Pol (PCR-211)
++ ++ 10-5 Standard PCR / optimized for minimal by-product formation
Routine and plate based PCR, automated pipetting
Hot Start Taq Polymerase
Hot Start Pol Apta+ (PCR-212)
++ +++ 10-5 High specificity PCR / high sensitivity PCR
High throughput PCR
Hot Start Pol Ab+ (PCR-213) ++ ++++ 10-5 High specificity PCR / high sensitivity PCR
Diagnostic PCR
High Fidelity Polymerase
Taq HiFi Pol (PCR-204)
+++ ++ 2 x 10-6 High fidelity PCR / long range PCR (> 30 kb)
Amplification of GC-rich / difficult templates
Pfu-X Pol (PCR-207) +++ +++ 2 x 10-7 High speed amplification with highest fidelity
Amplification of difficult and long templates
Ultra DNA Pol (PCR-391) +++ +++ n/a Robust and fast PCR amplification of templates from difficult matrices