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LM Agarose

Low melting Agarose

Cat. No. Amount Price (EUR) Buy / Note
PCR-277S 50 g 174,00 Add to Basket/Quote Add to Notepad
PCR-277L 5 x 50 g 696,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at ambient temperature

Shelf Life: 24 months

Form: fine, homogeneous, white powder

Description:
Low Melting (LM) Agaroses are derivatized by organic synthesis which generates methoxylate groups from the basic agarose structure. The main properties of these agaroses are their low melting and gelling temperatures when compared with standard agaroses.
LM Agarose is a recommended agarose for elecrophoresis of DNA fragments ≥ 10000 bp, tissue/cell culture and viral plaque assays. LM Agaroseis characterized by lower gel strength and highest clarity compared to standard agaroses.
The low melting temperature allows the recovery of undamaged nucleic acids below the denaturation temperature. The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where in-Gel manipulations can be performed without prior extraction of the DNA from the gel slice.

Specifications:

Moisture≤ 10 %
Ash≤ 0.4 %
Electroendosmosis≤ 0.12
Sulphate≤ 0.12 %
Clarity 1.5 % (NTU)≤ 4
Gel Strength 1.5 %≥ 500 g/cm2
Gelling Temperature 1.5 %24 - 28 °C
Melting Temperature 1.5 %≤ 65 °C

Functional tests

DNase/RNase activitynone detected
DNA Bindingvery low
DNA Resolutionfinaly resolved
Gel Backgroundvery low

Recommended Agarose Concentrations:

Size Range
(base pairs)
Final Agarose
Concentration [%]
1x TAE Buffer
5000 - 200000.60
300 - 150001.00
250 - 100001.25
200 - 50001.50
100 - 20001.75
80 - 10002.00