Low melting Agarose
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| PCR-277S | 50 g | 222,50 | Add to Basket/Quote Add to Notepad |
| PCR-277L | 5 x 50 g | 890,00 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months
Form: fine homogeneous powder
Color: white
Description:
Low Melting (LM) Agaroses are derivatized by organic synthesis which generates methoxylate groups from the basic agarose structure. The main properties of these agaroses are their low melting and gelling temperatures when compared with standard agaroses.
LM Agarose is a recommended agarose for elecrophoresis of DNA fragments ≥ 10000 bp, tissue/cell culture and viral plaque assays. LM Agaroseis characterized by lower gel strength and highest clarity compared to standard agaroses.
The low melting temperature allows the recovery of undamaged nucleic acids below the denaturation temperature. The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where in-Gel manipulations can be performed without prior extraction of the DNA from the gel slice.
Specifications:
| Moisture | ≤ 10 % |
| Ash | ≤ 0.4 % |
| Electroendosmosis | ≤ 0.12 |
| Sulphate | ≤ 0.12 % |
| Clarity 1.5 % (NTU) | ≤ 4 |
| Gel Strength 1.5 % | ≥ 500 g/cm2 |
| Gelling Temperature 1.5 % | 24 - 28 °C |
| Melting Temperature 1.5 % | ≤ 65 °C |
Functional tests
| DNase/RNase activity | none detected |
| DNA Binding | very low |
| DNA Resolution | finaly resolved |
| Gel Background | very low |
Recommended Agarose Concentrations:
| Size Range (base pairs) | Final Agarose Concentration [%] 1x TAE Buffer |
| 5000 - 20000 | 0.60 |
| 300 - 15000 | 1.00 |
| 250 - 10000 | 1.25 |
| 200 - 5000 | 1.50 |
| 100 - 2000 | 1.75 |
| 80 - 1000 | 2.00 |
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