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D-1 LE Agarose

general use Agarose

Cat. No. Amount Price (EUR) Buy / Note
PCR-269S 100 g 100,88 Add to Basket/Quote Add to Notepad
PCR-269L 500 g 390,24 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at ambient temperature

Shelf Life: 12 months

Form: fine, homogeneous, white powder

Applications:

  • Nucleic acid analytical and preparative electrophoresis
  • Blotting
  • Protein electrophoresis such as radial immunodiffusion

Description:
D-1 LE Agarose is a general use agarose for routine nucleic acid analytical and preparative electrophoresis of fragments between 200 - 20000 bp, blotting and protein electrophoresis. This agarose has a low electroendosmosis value (EEO), ensuring high electrophoretic mobility of nucleic acids. Because of the high gel strength, easy-to-handle gels can be prepared with this agarose at concentrations as low as 0.4 %. D-1 LE Agarose has an exceptionally low absorption of staining agents.

Specifications:

Moisture≤10 %
Ash≤0.4 %
Electroendosmosis0.05 - 0.13
Sulphate≤0.1 %
Clarity 1.5 % (NTU)≤3
Gel Strength 1 %≥1200 g/cm2
Gel Strength 1.5 %≥2500 g/cm2
Gelling Temperature 1.5 %36 ± 1.5 °C
Melting Temperature 1.5 %88 ± 1.5 °C

Functional tests

DNase/RNase activitynone detected
DNA resolution > 1000 bpfinaly resolved
Gel backgroundvery low

Recommended Agarose Concentrations:

Size Range
(base pairs)
Final Agarose
Concentration [%]
1x TAE Buffer
1000 - 200000.60
500 - 120000.80
300 - 80001.00
200 - 60001.20
100 - 30001.50
100 - 20002.00