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Crystal Buffer

Cat. No. Amount Price (EUR) Buy / Note
PCR-271 5 x 1,2 ml 10,60 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at 4 °C or -20 °C

Shelf Life: 12 months

Form: Liquid

Concentration: 10x conc.

Description:
Crystal PCR Buffer guarantees robust and reliable amplification results in almost all PCR applications. The buffer contains a well-balanced ratio of potassium-, ammonium- and magnesium-ions to ensure high specificity and minimal by-product formation without the need of additional optimization steps.

Content:
Crystal PCR Buffer (green cap)
200 mM Tris-HCl, KCl, (NH4)2SO4 and 20 mM MgCl2.

Optimization of MgCl2 concentration:
A final Mg2+ concentration of 2.0 mM is recommended for most PCR applications. For an individual optimization of the reaction buffer add 25 mM MgCl2 stock solution (PCR-266) as shown in the table below.

final MgCl2 conc.20 μl final assay volume50 μl final assay volume
2 mM--
3 mM0.8 μl2.0 μl
4 mM1.6 μl4.0 μl
5 mM2.4 μl6.0 μl