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Labeling Buffer

Cat. No. Amount Price (EUR) Buy / Note
PCR-263 5 x 1,2 ml 10,60 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at 4 °C or -20 °C

Shelf Life: 12 months

Form: liquid

Concentration: 10x conc.

Description:
Labeling Buffer is recommended for DNA labeling or mutagenesis. The PCR buffer system is specially optimized for incorporation of labeled or modified nucleotides into DNA. It gives superior results in a broad range of reaction conditions with most primer-template pairs but amplification may also tend to an increased unspecifity.

Content:
Labeling Buffer (green cap)
600 mM Tris-HCl (pH 8.8, 25 °C), 150 mM (NH4)2SO4, 20 mM MgCl2, 0.05 % Tween-20 and 0.05 % Nonidet P-40

Optimization of MgCl2 concentration:
A final Mg2+ concentration of 2.0 mM is recommended for most DNA labeling applications. However, if an individual Mg2+ optimization is essential add 25 mM MgCl2 stock solution (#PCR-266) as shown in the table below.

final MgCl2 conc.20 μl final assay volume50 μl final assay volume
2 mM--
3 mM0.8 μl2.0 μl
4 mM1.6 μl4.0 μl
5 mM2.4 μl6.0 μl

Related products: Mg2+ Stock, #PCR-266