Labeling Buffer

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at 4 °C or -20 °C

Shelf Life: 12 months

Form: liquid

Concentration: 10x conc.

Description:
Labeling Buffer is recommended for DNA labeling or mutagenesis. The PCR buffer system is specially optimized for incorporation of labeled or modified nucleotides into DNA. It gives superior results in a broad range of reaction conditions with most primer-template pairs but amplification may also tend to an increased unspecifity.

Content:
Labeling Buffer (green cap)
600 mM Tris-HCl (pH 8.8, 25 °C), 150 mM (NH₄)₂SO₄, 20 mM MgCl₂, 0.05 % Tween-20 and 0.05 % Nonidet P-40

Optimization of MgCl₂ concentration:
A final Mg²⁺ concentration of 2.0 mM is recommended for most DNA labeling applications. However, if an individual Mg²⁺ optimization is essential add 25 mM MgCl₂ stock solution (#PCR-266) as shown in the table below.

Related products: Mg²⁺ Stock, #PCR-266

Product Citations:
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