For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C or -20 °C
Shelf Life: 12 months
Concentration: 10x conc.
Labeling Buffer is recommended for DNA labeling or mutagenesis. The PCR buffer system is specially optimized for incorporation of labeled or modified nucleotides into DNA. It gives superior results in a broad range of reaction conditions with most primer-template pairs but amplification may also tend to an increased unspecifity.
Labeling Buffer (green cap)
600 mM Tris-HCl (pH 8.8, 25 °C), 150 mM (NH4)2SO4, 20 mM MgCl2, 0.05 % Tween-20 and 0.05 % Nonidet P-40
Optimization of MgCl2 concentration:
A final Mg2+ concentration of 2.0 mM is recommended for most DNA labeling applications. However, if an individual Mg2+ optimization is essential add 25 mM MgCl2 stock solution (#PCR-266) as shown in the table below.
|final MgCl2 conc.||20 μl final assay volume||50 μl final assay volume|
|3 mM||0.8 μl||2.0 μl|
|4 mM||1.6 μl||4.0 μl|
|5 mM||2.4 μl||6.0 μl|
Related products: Mg2+ Stock, #PCR-266
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