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KCl Buffer

Cat. No. Amount Price (EUR) Buy / Note
PCR-262 5 x 1,2 ml 10,60 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at 4 °C or -20 °C

Shelf Life: 12 months

Form: Liquid

Concentration: 10x conc.

Description:
PCR Buffer KCl is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation.

Content:
PCR Buffer KCl (green cap)
200 mM Tris-HCl (pH 8.5, 25 °C), 500 mM KCl and 15 mM MgCl2.

Optimization of MgCl2 concentration:
A final Mg2+ concentration of 1.5 mM is recommended for most PCR applications if using PCR Buffer KCl. For an individual optimization of the reaction buffer add 25 mM MgCl2 stock solution (PCR-266) as shown in the table below.

final MgCl2 conc.20 μl final assay volume50 μl final assay volume
1.5 mM--
2 mM0.4 μl1.0 μl
3 mM1.2 μl3.0 μl
4 mM2.0 μl5.0 μl
5 mM2.8 μl7.0 μl