Taq Pol mutant for incorporation of ddNTPs
Thermus species, recombinant, E. coli
For in vitro use only!
Unit Definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74 °C.
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Concentration: 5 units/μl
Sequencing Pol is a Taq polymerase mutant showing an enhanced efficiency for incorporation of ddNTPs. Its capability of incorporating ddNTPs and dNTPs at equal rates makes it the ideal choice for DNA cycle sequencing.
The enzyme replicates DNA at 74 °C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium and has a 5'→3' polymerisation-dependent exonuclease replacement activity only.
Sequencing Pol (red cap)
5 units/μl Sequencing Polymerase in storage buffer
Sequencing Buffer without MgCl2 (green cap)
500 mM Tris-HCl, pH 9.5 (25 °C)
MgCl2 Stock Solution (yellow cap)
25 mM MgCl2
Recommended 50 μl PCR assay:
|5 μl||10x Sequencing Buffer||blue cap|
|6 μl||MgCl2 stock|
|200 μM||each dNTP||-|
|0.2 - 1 μM||each Primer||-|
|2 - 50 ng||template DNA||-|
|Sequencing Pol||red cap|
|Fill up to 50 μl||PCR-grade water||-|
|MgCl2 Stock Solution||5 μl||6 μl||7 μl||8 μl|
|final MgCl2 conc.||2.5 mM||3 mM||3.5 mM||4 mM|
Availability Restriction: Exclusively distributed in Japan by Greiner BioOne