2',3'-Dideoxycytidine-5'-triphosphate, Lithium salt
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C9H16N3O12P3 (free acid)
Molecular Weight: 451.16 g/mol (free acid)
Purity: ≥ 98 % (HPLC), functionally tested by chain termination sequencing reactions
Form: clear aqueous solution
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 271 nm; ε 8.9 L mmol-1 cm-1 (Tris-HCl pH 7.5)
SNP: multiplexing by minisequencing
Multiplexed detection of alternatively spliced transcripts
Effect on reverse transcriptase of HIV-1
Molecular dynamic calculations of DNA-polymerase beta-ddCTP complex
Crystal structure with DNA- polymerase-I
 Gronlund et al. (2011) Direct detection of single-nucleotide polymorphisms in bacterial DNA by SNPtrap. Preparative Biochemistry and Biotechnology 41:166.
 Milani et al. (2006) Detection of alternatively spliced transcripts in leukemia cell lines by minisequencing on microarrays. Clinica Chemistry (Washington) 52:202.
 Anderson (2001) The molecular basis of inhibition and toxicity of modified cytosine analogues targetting HIV-1 reverse transcriptase. Antiviral Chemistry and Chemotherapy 12:13.
 Rittenhouse et al. (2003) Characterization of the active site of DNA polymerase beta by molecular dynamics and quantum chemical calculation. Proteins: Structure, Function and Genetics 53:667.
 Li et al. (2001) Crystal structures of ddATP-, ddTTP-, ddCTP-, and ddGTP-trapped ternary complex of Klentaq1: insights into nucleotide incorporation selectivity. Protein Science 10:1225.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463