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Fluorescent 50 bp DNA Ladder

50 bp to 500 bp log scale

ready-to-use, orange, green fluorescent

Cat. No. Amount Price (EUR) Buy / Note
M-233S 500 μl (105 ng/μl) 67,65 Add to Basket/Quote Add to Notepad
M-233L 5 x 500 μl (105 ng/μl) 270,60 Add to Basket/Quote Add to Notepad
5 μl loaded onto 3.0 % agarose
5 μl loaded onto 3.0 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
store dark
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid, orange, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 10 % (w/v) Glycerol, EvaGreenTM and Orange G

Concentration: 105 ng/μl

Description:
Fluorescent 50 bp DNA Ladder consists of 10 DNA fragments ranging from 50 bp to 500 bp. The prestained ladder is supplied in a ready-to-use format containing EvaGreenTM fluorescent DNA stain and an orange tracking dye. It allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest.
DNA log scale ladders are well suited for combination if small and large fragments should be analyzed on one gel.

Fluorescent DNA Stain:
DNA Ladders with Fluorescent Stain contain EvaGreenTM a fluorescent DNA intercalator dye specially developed for DNA analysis applications. High quantum yield and excellent stability makes EvaGreenTM the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide or SYBR® Green.
DNA Ladders with Fluorescent Stain are optimized for direct loading into unstained agarose gels and are recommended for use in combination with Gel Loading Buffer with DNA Stain (#PCR-274 - #PCR-276).
The ladders provide highest convenience during routine handling and avoid commonly used gel staining procedures with Ethidium Bromide or SYBR® Green.

Recommended Load:
5 μl per lane

DNA Fragments:
50, 100, 150, 200, 250, 300, 350, 400, 450, 500 bp