500 bp to 10 kb linear scale
ready-to-use, blue / blue, green fluorescent
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.
Shelf Life: 12 months
Form: liquid, blue, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 10 % (w/v) Glycerol, EvaGreenTM, Bromophenol Blue and Xylene Cyanol FF
Concentration: 130 ng/μl
Fluorescent High Range DNA Ladder consists of 12 DNA fragments ranging from 500 bp to 10 kb. It is designed to show virtually uniform spacing over a wide fragment range. The ladder allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest. The prestained fragment mix is supplied in ready-to-use format containing EvaGreenTM fluorescent DNA stain and blue tracking dyes.
Fluorescent DNA Stain:
DNA Ladders with Fluorescent Stain contain EvaGreenTM a fluorescent DNA intercalator dye specially developed for DNA analysis applications. High quantum yield and excellent stability makes EvaGreenTM the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide or SYBR® Green.
DNA Ladders with Fluorescent Stain are optimized for direct loading into unstained agarose gels and are recommended for use in combination with Gel Loading Buffer with DNA Stain (#PCR-274 - #PCR-276).
The ladders provide highest convenience during routine handling and avoid commonly used gel staining procedures with Ethidium Bromide or SYBR® Green.
5 μl per lane
0.5, 0.6, 0.8, 1, 1.5, 2, 3, 4, 5, 6, 8, 10 kb