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Fluorescent High Range DNA Ladder

500 bp to 10 kb linear scale

ready-to-use, blue / blue, green fluorescent

Cat. No. Amount Price (EUR) Buy / Note
M-224S 500 μl (130 ng/μl) 45,10 Add to Basket/Quote Add to Notepad
M-224L 5 x 500 μl (130 ng/μl) 180,40 Add to Basket/Quote Add to Notepad
5 μl loaded onto 0.6 % agarose
5 μl loaded onto 0.6 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
store dark
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid, blue, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 10 % (w/v) Glycerol, EvaGreenTM, Bromophenol Blue and Xylene Cyanol FF

Concentration: 130 ng/μl

Description:
Fluorescent High Range DNA Ladder consists of 12 DNA fragments ranging from 500 bp to 10 kb. It is designed to show virtually uniform spacing over a wide fragment range. The ladder allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest. The prestained fragment mix is supplied in ready-to-use format containing EvaGreenTM fluorescent DNA stain and blue tracking dyes.

Fluorescent DNA Stain:
DNA Ladders with Fluorescent Stain contain EvaGreenTM a fluorescent DNA intercalator dye specially developed for DNA analysis applications. High quantum yield and excellent stability makes EvaGreenTM the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide or SYBR® Green.
DNA Ladders with Fluorescent Stain are optimized for direct loading into unstained agarose gels and are recommended for use in combination with Gel Loading Buffer with DNA Stain (#PCR-274 - #PCR-276).
The ladders provide highest convenience during routine handling and avoid commonly used gel staining procedures with Ethidium Bromide or SYBR® Green.

Recommended Load:
5 μl per lane

DNA Fragments:
0.5, 0.6, 0.8, 1, 1.5, 2, 3, 4, 5, 6, 8, 10 kb