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Fluorescent Low Range DNA Ladder

50 bp to 1 kb linear scale

ready-to-use, orange / blue, green fluorescent

Cat. No. Amount Price (EUR) Buy / Note
M-222S 500 μl (120 ng/μl) 67,65 Add to Basket/Quote Add to Notepad
M-222L 5 x 500 μl (120 ng/μl) 270,60 Add to Basket/Quote Add to Notepad
5 μl loaded onto 3.0 % agarose
5 μl loaded onto 3.0 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
store dark
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid, green, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 8 % (w/v) Glycerol, Tartrazine and Xylene Cyanol FF

Concentration: 120 ng/μl

Description:
Fluorescent Low Range DNA Ladder consists of 11 DNA fragments ranging from 50 bp to 1 kb. It is designed to show virtually uniform spacing over a wide fragment range. The ladder allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest. The prestained fragment mix is supplied in ready-to-use format containing EvaGreenTM fluorescent DNA stain and orange / blue tracking dyes.

Fluorescent DNA Stain:
DNA Ladders with Fluorescent Stain contain EvaGreenTM a fluorescent DNA intercalator dye specially developed for DNA analysis applications. High quantum yield and excellent stability makes EvaGreenTM the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide or SYBR® Green.
DNA Ladders with Fluorescent Stain are optimized for direct loading into unstained agarose gels and are recommended for use in combination with Gel Loading Buffer with DNA Stain (#PCR-274 - #PCR-276).
The ladders provide highest convenience during routine handling and avoid commonly used gel staining procedures with Ethidium Bromide or SYBR® Green.

Recommended Load:
5 μl per lane

DNA Fragments:
50, 75, 100, 150, 200, 300, 400, 500, 600, 800, 1000 bp