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pUC19/ BseBI/ TaqI Digest

ready-to-use

Cat. No. Amount Price (EUR) Buy / Note
M-109S 100 μg 50,84 Add to Basket/Quote Add to Notepad
M-109L 5 x 100 μg 203,36 Add to Basket/Quote Add to Notepad
1 μg loaded onto 1.2 % agarose
1 μg loaded onto 1.2 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid (supplied in Jena Bioscience 6x Green Gel Loading Buffer (#PCR-254-gr))

Concentration: 400 ng/μl

Description:
The BseBI and TaqI digest of pUC19 DNA yields the following 9 discrete fragments (in base pairs): 2073, 1444, 736, 476, 288, 191, 121, 30, 13.

Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.

Recommended Load:
2.5 μl per lane

Preparation:
pUC19 DNA (2.686 bp) was completely digested by BseBI and TaqI, phenol/chloroform extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.