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pUC19/ BsiSI (HpaII) Digest

ready-to-use

Cat. No. Amount Price (EUR) Buy / Note
M-108S 100 μg 50,84 Add to Basket/Quote Add to Notepad
M-108L 5 x 100 μg 203,36 Add to Basket/Quote Add to Notepad
1 μg loaded onto 1.7 % agarose
1 μg loaded onto 1.7 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid (supplied in Jena Bioscience 6x Green Gel Loading Buffer (#PCR-254-gr))

Concentration: 400 ng/μl

Description:
The BsiSI digest of pUC19 DNA yields the following 13 discrete fragments (in base pairs): 501, 489, 404, 331, 242, 190, 147, 111, 110, 67, 34, 34, 26.

Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.

Recommended Load:
2.5 μl per lane

Preparation:
pUC19 DNA (2.686 bp) was completely digested by BsiSI, phenol/chloroform extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.