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pUC19/ BsiS I (Hpa II) Digest

ready-to-use

Product Cat. No. Amount Price (EUR) Buy / Note
pUC19/ BsiS I (Hpa II) Digest M-108S 100 μg 48,00 Add to Basket/Quote Add to Notepad
pUC19/ BsiS I (Hpa II) Digest M-108L 5 x 100 μg 192,00 Add to Basket/Quote Add to Notepad

1 μg loaded onto 1.7 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid (Supplied in Jena Bioscience 6x Green Gel Loading Buffer (PCR-254-gr).

Concentration: 400 ng/μl

Description:
The BsiS I digest of pUC19 DNA yields the following 13 discrete fragments (in base pairs): 501, 489, 404, 331, 242, 190, 147, 111, 110, 67, 34, 34, 26.

Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.

Recommended Load:
2.5 μl per lane

Preparation:
pUC19 DNA (2.686 bp) was completely digested by BsiS I, phenol/chloroform extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.